Open Journal Systems





Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture

VIEWS - 670 (Abstract) 304 (PDF)
Behnam Taidi, Guillaume Lebernede, Lothar Koch, Patrick Perre, Boris Chichkov

Abstract


Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been so far largely applied for printing mammalian cells. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bio-printing is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied by confocal microscopy and the colonies growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rates determination of the micro-colonies, are very promising for future studies of colony growth and development.

Keywords


Laser-induced forward transfer; bioprinting; printing of microorganisms; growth rates of micro-colonies; saccharomyces cerevisiae; chlorella vulgaris

Full Text:

PDF

References


Ringeisen B R, Karina R, Fitzgerald L A, et al. 2014, Printing soil: A single-step, high-throughput method to isolate micro-organisms and near-neighbour microbial consortia from a complex environmental sample. Methods in Ecology and Evolution, vol.6(1): 209–217. http://dx.doi.org/10.1111/2041-210X.12303

Walker D, Hill G, Wood S, et al. 2004, Agent-based computational modeling of wounded epithelial cell mono-layers. IEEE Transactions on Nanobioscience, vol.3(3): 153–163. http://dx.doi.org/10.1109/TNB.2004.833680

Emonet T, Macal C M, North M J, et al. 2005, Agent-Cell: A digital single-cell assay for bacterial chemotaxis. Bioinformatics, vol.21(11): 2714–2721. http://dx.doi.org/10.1093/bioinformatics/bti391

Zhang L, Wang Z, Sagotsky J A, et al. 2009, Multiscale agent-based cancer modeling. Journal of Mathematical Biology, vol.58(4–5): 545–559. http://dx.doi.org/10.1007/s00285-008-0211-1

Tang Y and Valocchi A J, 2013, An improved cellular automaton method to model multispecies biofilms. Water Research, vol.47(15): 5729–5742. http://dx.doi.org/10.1016/j.watres.2013.06.055

Gerken H G, Bryon D and Knoshaug E P, 2013, Enzymatic cell wall degradation of Chlorella vulgaris and other microalgae for biofuels production. Planta, vol.237(1): 239–253. http://dx.doi.org/10.1007/s00425-012-1765-0

Orlean P, 2012, Architecture and biosynthesis of the Saccharomyces cerevisiae cell wall. Genetics, vol.192(3):

–818. http://dx.doi.org/10.1534/genetics.112.144485

Martínez F and Orús M I, 1991, Interactions between glucose and inorganic carbon metabolism in Chlorella vulgaris strain UAM 101. Plant Physiology, vol.95(4): 1150–1155.

http://dx.doi.org/10.1104/pp.95.4.1150

Liang Y, Sarkany N and Cui Y, 2009, Biomass and lipid productivity of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnology Letters, vol.31(7): 1043–1049. http://dx.doi.org/10.1007/s10529-009-9975-7

Merico A, Sulo P, Piškur J, et al. 2007, Fermentative lifestyle in yeasts belonging to the Saccharomyces complex. The FEBS Journal, vol.274(4): 976–989. http://dx.doi.org/10.1111/j.1742-4658.2007.05645.x

Rosenfeld E, Beauvoit B, Blondin B, et al. 2003, Oxygen consumption by anaerobic Saccharomyces cerevisiae under enological conditions: Effect on fermentation kinetics. Applied and Environmental Microbiology, vol.69(1): 113–121. http://dx.doi.org/10.1128/AEM.69.1.113–121.2003

Ferris C J, Gilmore K J, Wallace G G, et al. 2013, Biofabrication: An overview of the approaches used for printing of living cells. Applied Microbiology and Biotechnology, vol.97(10): 4243–4258. http://dx.doi.org/10.1007/s00253-013-4853-6

Clément-Larosière B, Lopes F, Gonçalves A, et al. 2014, Carbon dioxide biofixation by Chlorella vulgaris at different CO2 concentrations and light intensities. Engineering in Life Sciences, vol.14(5): 509–519. http://dx.doi.org/10.1002/elsc.201200212

Koch L, Kuhn S, Sorg H, et al. 2010, Laser printing of skin cells and human stem cells. Tissue Engineering Part C: Methods, vol.16(5): 847–854. http://dx.doi.org/10.1089/ten.TEC.2009.0397

Unger C, Gruene M, Koch L, et al. 2011, Time-resolved imaging of hydrogel printing via laser-induced forward transfer. Applied Physics A, vol.103(2): 271–277. http://dx.doi.org/10.1007/s00339-010-6030-4

Gruene M, Unger C, Koch L, et al. 2011, Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting. Biomedical Engineering Online, vol.10: 19. http://dx.doi.org/10.1186/1475-925X-10-19

Monod J, 1949, The growth of bacterial cultures. Annual Review of Microbiology, vol.3: 371–394. http://dx.doi.org/10.1146/annurev.mi.03.100149.002103

Schiele N R, Corr D T, Huang Y, et al. 2010, Laser-based direct-write techniques for cell printing. Biofabrication, vol.2(3): 032001. http://dx.doi.org/10.1088/1758-5082/2/3/032001

Koch L, Deiwick A and Chichkov B, 2014, Laser-based 3D cell printing for tissue engineering. BioNanoMaterials, vol.15(3–4): 71–78. http://dx.doi.org/10.1515/bnm-2014-0005




DOI: http://dx.doi.org/10.18063/IJB.2016.02.001

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Behnam Taidi, Guillaume Lebernede, Lothar Koch, Patrick Perre, Boris Chichkov

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.