Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture

Behnam Taidi, Guillaume Lebernede, Lothar Koch, Patrick Perre, Boris Chichkov

Article ID: 73
Vol 2, Issue 2, 2016, Article identifier:37-43

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Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been so far largely applied for printing mammalian cells. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bio-printing is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied by confocal microscopy and the colonies growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rates determination of the micro-colonies, are very promising for future studies of colony growth and development.


Laser-induced forward transfer; bioprinting; printing of microorganisms; growth rates of micro-colonies; saccharomyces cerevisiae; chlorella vulgaris

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