Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Chanh Trung Nguyen, Van Thuy Duong, Chang Ho Hwang, Kyo-in Koo

Article ID: 557
Vol 8, Issue 3, 2022, Article identifier:

VIEWS - 136 (Abstract) 26 (PDF) 4 (Supp.File)

Abstract


Rapid construction of pre-vascular structure is highly desired for engineered thick tissue. However, angiogenesis in free-standing scaffold has been rarely reported because of limitation in growth factor (GF) supply into the scaffold. This study, for the 1st time, investigated angiogenic sprouting in free-standing two-vasculature-embedded scaffold with three different culture conditions and additional GFs. A two-core laminar flow device continuously extruded one vascular channel with human umbilical vein endothelial cells (HUVECs) and a 3 mg/ml type-1 collagen, one hollow channel, and a shell layer with 2% w/v gelatin-alginate (70:30) composite. Under the GF flowing condition, angiogenic sprouting from the HUVEC vessel had started since day 1 and gradually grew toward the hollow channel on day 10. Due to the medium flowing, the HUVECs showed elongated spindle-like morphology homogeneously. Their viability has been over 80% up to day 10. This approach could apply to vascular investigation, and drug discovery further, not only to the engineered thick tissue.


Keywords


Angiogenesis; Pre-vascularized tissue; Two-core vasculature; Gelatin-alginate; Free standing; Culture condition

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DOI: http://dx.doi.org/10.18063/ijb.v8i3.557

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