Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures

Shugang Hu, Zijie Meng, Junpeng Zhou, Yongwei Li, Yanwen Su, Qi Lei, Mao Mao, Xiaoli Qu, Jiankang He, Wei Wang

Article ID: 514
Vol 8, Issue 2, 2022, Article identifier:

VIEWS - 543 (Abstract) 255 (PDF) 31 (Supp.File)

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Abstract


Micro/sub-microscale fibrillar architectures of extracellular matrix play important roles in regulating cellular behaviors such as attachment, migration, and differentiation. However, the interactions between cells and organized micro/sub-microscale fibers have not been fully clarified yet. Here, the responses of MC3T3-E1 cells to electrohydrodynamic (EHD) printed scaffolds with microscale and/or sub-microscale fibrillar architectures were investigated to demonstrate their potential for bone tissue regeneration. Fibrillar scaffolds were EHD-fabricated with microscale (20.51 ± 1.70 μm) and/or sub-microscale (0.58 ± 0.51 μm) fibers in a controlled manner. The in vitro results showed that cells exhibited a 1.25-fold increase in initial attached cell number and 1.17-fold increase in vinculin expression on scaffolds with micro/sub-microscale fibers than that on scaffolds with pure microscale fibers. After 14 days of culture, the cells expressed 1.23 folds increase in collagen type I (COL-I) deposition compared with that on scaffolds with pure microscale fibers. These findings indicated that the EHD printed sub-microscale fibrous architectures can facilitate attachment and COL I secretion of MC3T3-E1 cells, which may provide a new insight to the design and fabrication of fibrous scaffolds for bone tissue engineering.


Keywords


Electrohydrodynamic printing, Micro/sub-microscale fibrous architectures, MC3T3-E1, Cell-scaffold interaction, Bone tissue engineering

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DOI: http://dx.doi.org/10.18063/ijb.v8i2.514

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