Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs

Sanjairaj Vijayavenkataraman, Novelia Vialli, Jerry Y.H. Fuh, Wen Feng Lu

Article ID: 229
Vol 5, Issue 2.1, 2019, Article identifier:229

VIEWS - 3337 (Abstract) 513 (PDF)


Bioprinting is increasingly being used for fabrication of engineered tissues for regenerative medicine, drug testing, and other biomedical applications. The success of this technology lies with the development of suitable bioinks and hydrogels that are specific to the intended tissue application. For applications such as neural tissue engineering, conductivity plays an important role in determining the neural differentiation and neural tissue regeneration. Although several conductive hydrogels based on metal nanoparticles (NPs) such as gold and silver, carbon-based materials such as graphene and carbon nanotubes and conducting polymers such as polypyrrole (PPy) and polyaniline were used, they possess several disadvantages. The long-term cytotoxicity of metal nanoparticles (NPs) and carbon-based materials restricts their use in regenerative medicine. The conductive polymers, on the other hand, are non-biodegradable and possess weak mechanical properties limiting their printability into three-dimensional constructs. The aim of this study is to develop a biodegradable, conductive, and printable hydrogel based on collagen and a block copolymer of PPy and polycaprolactone (PCL) (PPy-block-poly(caprolactone) [PPy-b-PCL]) for bioprinting of neural tissue constructs. The printability, including the influence of the printing speed and material flow rate on the printed fiber width; rheological properties; and cytotoxicity of these hydrogels were studied. The results prove that the collagen/PPy-b-PCL hydrogels possessed better printability and biocompatibility. Thus, the collagen/PPy-b-PCL hydrogels reported this study has the potential to be used in the bioprinting of neural tissue constructs for the repair of damaged neural tissues and drug testing or precision medicine applications.


Three-dimensional printing; Tissue engineering scaffolds; Peripheral nerve injury; Nerve guide conduit; Conductive scaffolds; Stem cells

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Vijayavenkataraman S, Yan WC, Lu WF, et al., 2018, 3D Bioprinting of Tissues and Organs for Regenerative Medicine. Advanced Drug Delivery Reviews, 132:296-332. DOI 10.1016/j.addr.2018.07.004.

Merceron TK, Murphy SV, 2015, Hydrogels for 3D Bioprinting Applications, Essentials of 3D Biofabrication and Translation. San Diego: Elsevier, pp. 249-70. DOI 10.1016/b978-0-12-800972-7.00014-1.

Skardal A, Devarasetty M, Kang HW, et al., 2016, Bioprinting Cellularized Constructs using a Tissue-specific Hydrogel Bioink. Journal of Visualized Experiments, 110:e53606. DOI 10.3791/53606.

Vijayavenkataraman S, Thaharah S, Zhang S, et al., 2019, Electrohydrodynamic Jet 3D-printed PCL/PAA Conductive Scaffolds with Tunable Biodegradability as Nerve Guide Conduits (NGCs) for Peripheral Nerve Injury Repair. Materials and Design, 162:71-184. DOI 10.1016/j. matdes.2018.11.044.

Vijayavenkataraman S, Thaharah S, Zhang S, et al., 2018, 3D-Printed PCL/rGO Conductive Scaffolds for Peripheral Nerve Injury Repair. Artificial Organs, 43(5):515-23. DOI 10.1111/aor.13360.

Xing R, Liu K, Jiao T, et al., 2016, An Injectable Self- Assembling Collagen Gold Hybrid Hydrogel for Combinatorial Antitumor Photothermal/Photodynamic Therapy. Advanced Materials, 28(19):3669-76. DOI 10.1002/ adma.201600284.

Xu L, Li X, Takemura T, et al., 2012, Genotoxicity and Molecular Response of Silver Nanoparticle (NP)-based Hydrogel. Journal of Nanobiotechnology, 10(1):16. DOI 10.1186/1477-3155-10-16.

Zare M, Ramezani Z, Rahbar N, 2016, Development of Zirconia Nanoparticles-decorated Calcium Alginate Hydrogel Fibers for Extraction of Organophosphorous Pesticides from Water and Juice Samples: Facile Synthesis and Application with Elimination of Matrix Effects. Journal of Chromatography A, 1473:28-37. DOI 10.1016/j. chroma.2016.10.071.

Paquet C, de Haan HW, Leek DM, et al., 2011, Simard, Clusters of Superparamagnetic Iron Oxide Nanoparticles Encapsulated in a Hydrogel: A Particle Architecture Generating a Synergistic Enhancement of the T2 Relaxation. ACS Nano, 5(4):3104-12. DOI 10.1021/nn2002272.

Min J, Patel M, Koh WG, 2018, Incorporation of Conductive Materials into Hydrogels for Tissue Engineering Applications. Polymers, 10(10):1078. DOI 10.3390/polym10101078.

Lee WC, Lim CHY, Shi H, et al., 2011, Origin of Enhanced Stem Cell Growth and Differentiation on Graphene and Graphene Oxide. ACS Nano, 5(9):7334-41. DOI 10.1021/ nn202190c.

Jing X, Mi HY, Napiwocki BN, et al., 2017, Turng, Mussel-inspired Electroactive Chitosan/Graphene Oxide Composite Hydrogel with Rapid Self-healing and Recovery Behavior for Tissue Engineering. Carbon, 125:557-570. DOI 10.1016/j. carbon.2017.09.071.

Shin SR, Zihlmann C, Akbari M, et al., 2016, Reduced Graphene Oxide-gelMA Hybrid Hydrogels as Scaffolds for Cardiac Tissue Engineering. Small, 12(27):3677-89. DOI 10.1002/smll.201600178.

Sun H, Zhou J, Huang Z, et al., 2017, Carbon Nanotube-incorporated Collagen Hydrogels Improve Cell Alignment and the Performance of Cardiac Constructs. International Journal of Nanomedicine, 12:3109. DOI 10.2147/ijn. s128030.

Yang S, Jang L, Kim S, et al., 2016, Polypyrrole/Alginate Hybrid Hydrogels: Electrically Conductive and Soft Biomaterials for Human Mesenchymal Stem Cell Culture and Potential Neural Tissue Engineering Applications. Macromolecular Bioscience, 16(11):1653-61. DOI 10.1002/ mabi.201600148.

Srisuk P, Berti FV, da Silva LP, et al., 2018, Electroactive Gellan Gum/Polyaniline Spongy-like Hydrogels. ACS Biomaterials Science and Engineering, 4(5):1779-87. DOI 10.1021/acsbiomaterials.7b00917.

Mawad D, Artzy-Schnirman A, Tonkin J, et al., 2016, Electroconductive Hydrogel Based on Functional Poly (Ethylenedioxy Thiophene). Chemistry of Materials, 28(17):6080-8. DOI 10.1021/acs.chemmater.6b01298.

Ribeiro A, Blokzijl MM, Levato R, et al., 2017, Assessing Bioink Shape Fidelity to Aid Material Development in 3D Bioprinting. Biofabrication, 10(1):14102. DOI 10.1088/1758- 5090/aa90e2.

Engler AJ, Sen S, Sweeney HL, et al., 2006, Matrix Elasticity Directs Stem Cell Lineage Specification. Cell, 126(4):677-89. DOI 10.1016/j.cell.2006.06.044.

Lee C.H, Singla A, Lee Y, 2001, Biomedical Applications of Collagen. International Journal of Pharmaceutics, 221(1-2):1-22.

Levental KR, Yu H, Kass L, et al., 2009, Matrix Crosslinking Forces Tumor Progression by Enhancing Integrin Signaling. Cell, 139(5):891-906. DOI 10.1016/j.cell.2009.10.027.

Lee C, Grodzinsky A, Spector M, 2001, The Effects of Cross-linking of Collagen-glycosaminoglycan Scaffolds on Compressive Stiffness, Chondrocyte-mediated Contraction, Proliferation and Biosynthesis. Biomaterials, 22(23):3145- 54. DOI 10.1016/s0142-9612(01)00067-9.

Roberts J, Martens P, 2016, Engineering Biosynthetic Cell Encapsulation Systems. In: Biosynthetic Polymers for Medical Applications. San Diego: Elsevier, pp. 205-39. DOI 10.1016/b978-1-78242-105-4.00009-2.

Lau YKI, Gobin AM, West JL, 2006, Overexpression of Lysyl Oxidase to Increase Matrix Crosslinking and Improve Tissue Strength in Dermal Wound Healing. Annals of Biomedical Engineering, 34(8):1239-46. DOI 10.1007/s10439-006- 9130-8.

Shi Z, Gao H, Feng J, et al., 2014, In situ Synthesis of Robust Conductive Cellulose/Polypyrrole Composite Aerogels and their Potential Application in Nerve Regeneration. Angewandte Chemie International Edition, 53(21):5380-4. DOI 10.1002/anie.201402751.

Bu Y, Xu HX, Li X, et al., 2018, A Conductive Sodium Alginate and Carboxymethyl Chitosan Hydrogel Doped with Polypyrrole for Peripheral Nerve Regeneration. RSC Advances, 8(20):10806-17. DOI 10.1039/c8ra01059e.

Javadi M, Gu Q, Naficy S, et al., 2018, Conductive Tough Hydrogel for Bioapplications. Macromolecular Bioscience, 18(2): 1700270. DOI 10.1002/mabi.201700270.

Guo B, Ma PX, 2018, Conducting Polymers for Tissue Engineering. Biomacromolecules, 19(6):1764-82.



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