Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures

Authors

  • Shugang Hu Department of Bone and Joint Surgery, the Second Affiliated Hospital of Xian Jiaotong University, Xian Shaanxi, 710004, People’s Republic of China
  • Zijie Meng State key laboratory for manufacturing systems engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, People’s Republic of China;NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, 710049, China
  • Junpeng Zhou Department of Bone and Joint Surgery, the Second Affiliated Hospital of Xian Jiaotong University, Xian Shaanxi, 710004, People’s Republic of China
  • Yongwei Li Department of Bone and Joint Surgery, the Second Affiliated Hospital of Xian Jiaotong University, Xian Shaanxi, 710004, People’s Republic of China
  • Yanwen Su State key laboratory for manufacturing systems engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, People’s Republic of China;NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, 710049, China
  • Qi Lei State key laboratory for manufacturing systems engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, People’s Republic of China;NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, 710049, China
  • Mao Mao State key laboratory for manufacturing systems engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, People’s Republic of China;NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, 710049, China
  • Xiaoli Qu State key laboratory for manufacturing systems engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, People’s Republic of China;NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, 710049, China
  • Jiankang He State key laboratory for manufacturing systems engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, People’s Republic of China;NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, 710049, China
  • Wei Wang Department of Bone and Joint Surgery, the Second Affiliated Hospital of Xian Jiaotong University, Xian Shaanxi, 710004, People’s Republic of China

DOI:

https://doi.org/10.18063/ijb.v8i2.514

Keywords:

Electrohydrodynamic printing, Micro/sub-microscale fibrous architectures, MC3T3-E1, Cell-scaffold interaction, Bone tissue engineering

Abstract

Micro/sub-microscale fibrillar architectures of extracellular matrix play important roles in regulating cellular behaviors such as attachment, migration, and differentiation. However, the interactions between cells and organized micro/ sub-microscale fibers have not been fully clarified yet. Here, the responses of MC3T3-E1 cells to electrohydrodynamic (EHD) printed scaffolds with microscale and/or sub-microscale fibrillar architectures were investigated to demonstrate their potential for bone tissue regeneration. Fibrillar scaffolds were EHD-fabricated with microscale (20.51 ± 1.70 μm) and/or sub-microscale (0.58 ± 0.51 μm) fibers in a controlled manner. The in vitro results showed that cells exhibited a 1.25-fold increase in initial attached cell number and 1.17-fold increase in vinculin expression on scaffolds with micro/sub-microscale fibers than that on scaffolds with pure microscale fibers. After 14 days of culture, the cells expressed 1.23 folds increase in collagen type I (COL-I) deposition compared with that on scaffolds with pure microscale fibers. These findings indicated that the EHD printed sub-microscale fibrous architectures can facilitate attachment and COL I secretion of MC3T3-E1 cells, which may provide a new insight to the design and fabrication of fibrous scaffolds for bone tissue engineering.

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Published

2022-02-11

Issue

Vol. 8 No. 2 (2022): International Journal of Bioprinting

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Research Article

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