Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering


  • Krishna C. R. Kolan Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, Rolla, Missouri, USA
  • Julie A. Semon Department of Biological Sciences, Missouri University of Science and Technology, Rolla, Missouri, USA
  • Bradley Bromet Department of Biological Sciences, Missouri University of Science and Technology, Rolla, Missouri, USA
  • Delbert E. Day Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, Missouri, USA
  • Ming C. Leu Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, Rolla, Missouri, USA



Bioprinting, Alginate-gelatin bioink, Borate bioactive glass, Human adipose-derived stem cells Polymer/bioactive glass composite


Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin (Alg-Gel) hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds with 12 × 12 × 1 mm3 in overall dimensions are physically characterized, and the glass dissolution from PCL/glass composite over a period of 28 days is studied. Alg-Gel composite hydrogel is used as a bioink to suspend ASCs, and scaffolds are then bioprinted in different configurations: Bioink only, PCL+bioink, and PCL/glass+bioink, to investigate ASC viability. The results indicate the feasibility of the solvent-based bioprinting process to fabricate 3D cellularized scaffolds with more than 80% viability on day 0. The decrease in viability after 7 days due to glass concentration and static culture conditions is discussed. The feasibility of modifying Alg-Gel with 13-93B3 glass for bioprinting is also investigated, and the results are discussed.


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