Discovering the Latest Scientific Pathways on Tissue Spheroids: Opportunities to Innovate

Marisela Rodriguez-Salvador, Baruc Emet Perez-Benitez, Karen Marcela Padilla-Aguirre

Article ID: 331
Vol 7, Issue 1, 2021, Article identifier:331

VIEWS - 256 (Abstract) 56 (PDF)


Tissue spheroids consist of a three-dimensional model of cells which is capable of imitating the complicated composition of healthy and unhealthy human tissue. Due to their unique properties, they can bring innovative solutions to tissue engineering and regenerative medicine, where they can be used as building blocks for the formation of organ and tissue models used in drug experimentation. Considering the rapid transformation of the health industry, it is crucial to assess the research dynamics of this field to support the development of innovative applications. In this research, a scientometric analysis was performed as part of a Competitive Technology Intelligence methodology, to determine the main applications of tissue spheroids. Papers from Scopus and Web of Science published between 2000 and 2019 were organized and analyzed. In total, 868 scientific publications were identified, and four main categories of application were determined. Main subject areas, countries, cities, authors, journals, and institutions were established. In addition, a cluster analysis was performed to determine networks of collaborations between institutions and authors. This article provides insights into the applications of cell aggregates and the research dynamics of this field, which can help in the decision-making process to incorporate emerging and innovative technologies in the health industry.


Scientometric analysis; Competitive technology intelligence; Bioprinting; Cell aggregates; Bioink

Full Text:



Donderwinkel I, Hest JC, Cameron NR, 2017, Bio-inks for 3D Bio-Printing: Recent Advances and Future Prospects. Polym. Chem , 8:4451–71.

Ngo TD, Kashami A, Imbalzano G, et al., 2018, Additive Manufacturing (3D Printing): A Review of Materials, Methods, Applications and Challenges. Compos B Eng, 143:172–96.

Ashammakhi N, Ahadian S, Xu C, et al., 2019, Bioinks and Bio-Printing Technologies to Make Heterogeneous and Biomimetic Tissue Constructs. Mater Today Bio, 1:100008.

Schwab A, Levato R, D’Este M, et al., 2020, Printability and Shape Fidelity of Bioinks in 3D Bioprinting. Chem Rev, 120:11028–55.

Ng WL, Chua CK, Shen YF, 2019, Print Me An Organ! Why We Are Not There Yet. Prog Polym Sci, 97:101145.

Ji S, Guvendiren M, 2017, Recent Advances in Bioink Design for 3D Bio-Printing of Tissues and Organs. Front Bioeng Biotechnol, 5:23.

Lee JM, Ng WL, Yeong WY, 2019, Resolution and Shape in Bio-Printing: Strategizing Towards Complex Tissue and Organ Printing. Appl Phys Rev, 6:11307.

Colosi C, Shin SR, Manoharan V, et al., 2016, Microfluidic Bio-Printing of Heterogeneous 3D Tissue Constructs Using Low-Viscosity Bioink. Adv Mater, 28:677–84.

Hospodiuk M, Dey M, Sosnoski D, et al., 2017, The Bioink: A Comprehensive Review on Bio-Printable Materials. Biotechnol Adv, 35:217–39.

Rezende RA, Pereira FD, Kasyanov V, et at., 2013, Scalable biofabrication of tissue spheroids for organ printing. In: Procedia CIRP. Vol. 5. Amsterdam, Elsevier. pp276–81.

Sriphutkiat Y, Kasetsirikul S, Zhou Y, 2018, Formation of Cell Spheroids Using Standing Surface Acoustic Wave (SSAW). Int J Bioprint, 4:130.

Costa EC, Melo-Diogo DD, Moreira AF, et al., 2017, Spheroids Formation on Non-Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches. Biotechnol J, 13:1002.

Gopinathan J, Noh I, 2018, Recent Trends in Bio-Inks for 3D Printing. Biomater Res, 22:11.

Murphy SV, Atala A, 2014, 3D Bio-Printing of Tissues and Organs. Nat Biotechnol, 32:773–85.

Jose RR, Rodriguez MJ, Dixon TA, et al., 2016, Evolution of Bio-Inks and Additive Manufacturing Technologies for 3D Bio-Printing. ACS Biomater Sci Eng, 2:1662-78.

Mehesz AN, Brown J, Hajdu Z, et al., 2011, Scalable Robotic Bio-Fabrication of Tissue Spheroids. Biofabrication, 3:025002.

Peng W, Unutmaz D, Ozbolat IT, 2016, Bio-Printing towards Physiologically Relevant Tissue Models for Pharmaceutics. Trends Biotechnol, 34:722–32.

Toit AS, 2015, Competitive Intelligence Research: An Investigation of Trends in the Literature. J Intell Stud Bus, 5:14–21.

Rodríguez-Salvador M, Villarreal-Garza D, Álvarez MM, et al., 2019, Analysis of the Knowledge Landscape of Three-Dimensional Bio-Printing in Latin America. Int J Bioprint, 5:240.

Elsevier, 2019, Scopus. Available from: [Last accessed on 2020 Oct 20].

Clarivate Analytics, 2019, Databases. Available from: [Last accessed on 2020 Oct 20].

Machino R, Matsumoto K, Taniguchi D, et al., 2019, Replacement of Rat Tracheas by Layered, Trachea-Like, Scaffold-Free Structures of Human Cells Using a Bio-3D Printing System. Adv Healthc Mater, 8:1800983.

Daly AC, Kelly DJ, 2019, Bio-Fabrication of Spatially Organised Tissues by Directing the Growth of Cellular Spheroids within 3D Printed Polymeric Microchambers. Biomaterials, 197:194–206.

Anada T, Pan CC, Stahl AM, et al., 2019, Vascularized Bone-Mimetic Hydrogel Constructs by 3D Bioprinting to Promote Osteogenesis and Angiogenesis. Int J Mol Sci, 20:1096.

Lee C, Abelseth E, de la Vega L, et al., 2019, Bioprinting a Novel Glioblastoma Tumor Model Using a Fibrin-Based Bio-Ink for Drug Screening. Mater Today Chem, 12:78–84.



  • There are currently no refbacks.

Copyright (c) 2021 Marisela Rodriguez-Salvador

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.