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Preparation and printability of ultrashort self-assembling peptide nanoparticles

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Sarah Ghalayini, Hepi Hari Susapto, Sophie Hall, Kowther Kahin, Charlotte Hauser


Nanoparticles (NPs) have left their mark on the field of bioengineering. Fabricated from metallic, magnetic, and metal oxide materials, their applications include drug delivery, bioimaging, and cell labeling. However, as they enter the body, the question remains – where do they go after fulfilling their designated function? As most materials used to produce NPs are not naturally found in the body, they are not biodegradable and may accumulate overtime. There is a lack of comprehensive, long-term studies assessing the biodistribution of non-biodegradable NPs for even the most widely studied NPs. There is a clear need for NPs produced from natural materials capable of degradation in vivo. As peptides exist naturally within the human body, their non-toxic and biocompatible nature comes as no surprise. Ultrashort peptides are aliphatic peptides designed with three to seven amino acids capable of self-assembling into helical fibers within macromolecular structures. Using a microfluidics flow-focusing approach, we produced different peptide-based NPs that were then three-dimensional (3D) printed with our novel printer setup. Herein, we describe the preparation method of NPs from ultrashort self-assembling peptides and their morphology in both manual and 3D-printed hydrogels, thus suggesting that peptide NPs are capable of withstanding the stresses involved in the printing process


Nanoparticles, ultrashort peptides, self-assembly, microfluidics, biomaterials

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Bobo D, Robinson KJ, Islam J, et al., 2016, Nanoparticle-Based

Medicines: A Review of Materials and Clinical Trials to Date. Pharm Res, 33:2373-87. DOI 10.1007/s11095-016-1958-5.

Smith AW, Nie S, 2010, Semiconductor Nanocrystals. Acc Chem Res, 43:190-200. DOI 10.1021/ar9001069.

Jain PK, Huang X, El-Sayed IH, et al., 2007, Review of Some Interesting Surface Plasmon Resonance-enhanced Properties of Noble Metal Nanoparticles and Their Applications to Biosystems. Plasmonics, 2:107-18. DOI 10.1007/s11468-007-9031-1.

Mikhaylova M, Kim DK, Bobrysheva N, et al., 2004, Superparamagnetism of Magnetite Nanoparticles: Dependence on Surface Modification. Langmuir, 20:2472-7. DOI 10.1021/la035648e.

Semmler-Behnke M, Kreyling WG, Lipka J, et al., 2008, Biodistribution of 1.4 and 18-nm Gold Particles in Rats. Small, 4:2108-11. DOI 10.1002/smll.200800922.

De Jong WH, Hagens WI, Krystek P, et al., 2008, Particle Size-dependent Organ Distribution of Gold Nanoparticles after Intravenous Administration. Biomaterials, 29:1912-9. DOI 10.1016/j.biomaterials.2007.12.037.

Goel R, Shah N, Visaria R, et al., 2009, Biodistribution of TNF-alpha-coated Gold Nanoparticles in an in vivo Model System. Nanomedicine, 4:401-10. DOI 10.2217/nnm.09.21.

Zhang G, Yang Z, Lu W, et al., 2009, Influence of Anchoring

Ligands and Particle Size on the Colloidal Stability and in vivo Biodistribution of Polyethylene Glycol-coated Gold Nanoparticles in Tumor-xenografted Mice. Biomaterials, 30:1928-36. DOI 10.1016/j.biomaterials.2008.12.038.

Sun L, Fan Z, Wang Y, et al., 2015, Tunable Synthesis of Selfassembled

Cyclic Peptide Nanotubes and Nanoparticles. Soft Matter, 11:3822. DOI 10.1039/c5sm00533g.

Habibi N, Kamaly N, Memic A, et al., 2016, Self-assembled

Peptide-based Nanostructures: Smart Nanomaterials Toward Targeted Drug Delivery. Nano Today, 11:41-60. DOI 10.1016/j.nanotod.2016.02.004.

DeFrates K, Markiewicz T, Gallo P, et al., 2018, Protein Polymer-Based Nanoparticles: Fabrication and Medical Applications. Int J Mol Sci, 19:1717-36. DOI 10.3390/ ijms19061717.

Lammel AS, Xiao H, Park SH, et al., 2010, Controlling Silk Fibroin Particle Features for Drug Delivery. Biomaterials, 31:4583-91. DOI 10.1016/j.biomaterials.2010.02.024.

Oliveira A, Guimarães K, Cerize N, et al., 2013, Nano Spray Drying as an Innovative Technology for Encapsulating Hydrophilic Active Pharmaceutical Ingredients (API). J Nanomed Nanotechnol, 4:6. DOI 10.4172/2157-7439.1000186.

Haas PA, 1992, Formation of Uniform Liquid Drops by Application of Vibration to Laminar Jets. Ind Eng Chem Res, 31:959-67. DOI 10.1021/ie00003a043.

Yadav TP, Yadav RM, Singh D, 2012, Mechanical Milling: A Top down Approach for the Synthesis of Nanomaterials and Nanocomposites. Nanosci Nanotechnol, 2:22-48. DOI 10.5923/j.nn.20120203.01.

Aiertza MK, Odriozola I, Cabañero G, et al., 2011, Singlechain

Polymer Nanoparticles. Cell Mol Life Sci, 69:337-46. DOI 10.1007/s00018-011-0852.

Yoon J, Kwag J, Shin TJ, et al., 2014, Nanoparticles of Conjugated Polymers Prepared from Phase-Separated Films of Phospholipids and Polymers for Biomedical Applications. Adv Mater, 26:4559-64. DOI 10.1002/adma.201400906.

Yang Y, Khoe U, Wang X, et al., 2009, Designer Selfassembling

Peptide Nanomaterials. Nano Today, 4:193-210. DOI 10.1016/j.nanotod.2009.02.009.

Karnik R, Gu F, Basto P, et al., 2008, Microfluidic Platform for Controlled Synthesis of Polymeric Nanoparticles. Nano Lett, 8:2906-12. DOI 10.1021/nl801736q.

Ni M, Zhuo S, Iliescu C, et al., 2019, Self-assembling Amyloid-like Peptides as Exogenous Second Harmonic Probes for Bioimaging Applications. J Biophotonics, 4:e201900065. DOI 10.1002/jbio.201900065.

Ni M, Tresset G, Iliescu C, et al., 2019, Microfluidics-assisted

Self-assembly of Ultrashort Peptides and their Application as Theranostic Nanoparticles.

Arab W, Rauf S, Al-Harbi O, et al., 2018, Novel Ultrashort Self-assembling Peptide Bioinks for 3D Culture of Muscle Myoblast Cells. Int J Bioprinting, 4(2):129. DOI 10.18063/ ijb.v4i2.129.

Arab WT, Niyas AM, Seferji K, et al., 2018, Evaluation of Peptide Nanogels for Accelerated Wound Healing in Normal Micropigs. Front Nanosci Nanotech, 4(4):1-9. DOI 10.15761/ fnn.1000173.

Arab WT, Kahin K, Khan Z, et al., 2019, Exploring Nanofibrous Self-assembling Peptide Hydrogels using Mouse Myoblast Cells for 3D Bioprinting and Tissue Engineering Applications. Int J Bioprinting, 5(2):198. DOI 10.18063/ijb. v5i2.198.

Reithofer MR, Lakshmanan A, Ping ATK, et al., 2014, In situ Synthesis of Size-controlled, Stable Silver Nanoparticles within Ultrashort Peptide Hydrogels and their Anti-bacterial Properties. Biomaterials, 35:7535-42. DOI 10.1016/j.biomaterials.2014.04.102.

Loo Y, Lakshmanan A, Ni M, et al., 2010, Peptide Bioink: Self-assembling Nanofibrous Scaffolds for 3d Organotypic Cultures. Nano Lett, 15:6919-25. DOI 10.1021/acs. nanolett.5b02859.

Sundaramurthi D, Rauf S, Hauser CAE, 2016, 3D Bioprinting

Technology for Regenerative Medicine Applications. Int J Bioprinting, 2:9-16. DOI 10.18063/ijb.2016.02.010.

Loo Y, Hauser CAE, 2016, Bioprinting Synthetic Self-assembling Peptide Hydrogels for Biomedical Applications. Biomed Mater, 11:114103. DOI 10.1088/1748-6041/11/1/014103.

Khan Z, Kahin K, Rauf S, et al., 2019, Optimization of a 3D Bioprinting Process using Ultrashort Peptide Bioinks. Int J Bioprinting, 5(1):173. DOI 10.18063/ijb.v5i1.173.

Kahin K, Khan Z, Albagami M, et al., 2019, Development of a Robotic 3D Bioprinting and Microfluidic Pumping System for Tissue and Organ Engineering. Proc SPIE, 17:108750Q. DOI 10.1117/12.2507237.

Zhao X, Pan F, Xu H, et al., 2010, Molecular Self-assembly

and Applications of Designer Peptide Amphiphiles. Chem Soc Rev, 39:3480-98.

Versluis F, Marsden HR, Kros A, 2010, Power Struggles in Peptide-amphiphile Nanostructures. Chem Soc Rev, 39:3434-44. DOI 10.1039/b919446k.

Lakshmanan A, Hauser CAE, 2011, Ultrasmall Peptides Self-assemble into Diverse Nanostructures: Morphological Evaluation and Potential Implications. Int J Mol Sci, 12:5736-46. DOI 10.3390/ijms12095736.

Loo Y, Zhang S, Hauser CAE, 2012, From Short Peptides to Nanofibers to Macromolecular Assemblies in Biomedicine. Biotech Adv, 30:593-603. DOI 10.1016/j. biotechadv.2011.10.004.

Cui H, Webber M, Stupp S, 2010, Self-Assembly of Peptide Amphiphiles: From Molecules to Nanostrucutres to Biomaterials. Biopolymers, 94:1-18. DOI 10.1002/ bip.21328.

Lakshmanan A, Zhang S, Hauser CAE, 2012, Short Selfassembling

Peptides as Building Blocks for Modern Nanodevices. Trends Biotech, 30:155-65. DOI 10.1016/j. tibtech.2011.11.001.

Hauser CAE, Zhang S, 2010, Designer Self-assembling Peptide Nanofiber Biological Materials. Chem Soc Rev, 39:2780-90. DOI 10.1039/b921448h.

Reithofer MR, Chan KH, Lakshmanan A, et al., 2014, Ligation of Anti-cancer Drugs to Self-assembling Ultrashort Peptides by Click Chemistry for Localized Therapy. Chem Sci, 5:625-30. DOI 10.1039/c3sc51930a.

Liu X, Huang J, Chen T, et al., 2008, Yamanaka Factors Critically Regulate the Developmental Signaling Network in Mouse Embryonic Stem Cells. Cell Res, 18(12):1177-89. DOI 10.1038/cr.2008.309.



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Copyright (c) 2019 Sarah Ghalayini, Hepi Hari Susapto, Sophie Hall, Kowther Kahin, Charlotte Hauser

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