A methodology to develop a vascular geometry for in vitro cell culture using additive manufacturing

Laurene Lenoir, Frederic Segonds, Kim-Anh Nguyen, Pablo Bartolucci

Article ID: 238
Vol 5, Issue 2, 2019, Article identifier:238

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Today, additive manufacturing (AM) is implemented in medical industry and profoundly revolutionizes this area. This approach consists of producing parts by additions of layers of successive materials and offers advantages in terms of rapidity, complexity of parts, competitive costs that can be exploited and can lead to a significant advancement in biological research. Everything becomes technically feasible and gives way to a “techno-centered” approach. Many parameters must be controlled in this field, so it is necessary to be guided for the development of such a product. This article aims to present a state of the art of existing design methodologies focused on AM to create medical devices. Finally, a development method is proposed that consists of producing vascular geometry using AM, based on patient data, designed for cell culture in vitro studies.


Innovation, Design, Additive Manufacturing, Biology, Medical device

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Lorec A, 2017, Un Vecteur Made in CEA Contre la Drépanocytose. Les Défis Du Cea, 24(213):8-9.

Rees DC, Williams TN, Gladwin MT, 2010, Sicklecell Disease. Lancet, 376(9757):2018-31. DOI 10.1016/ S0140-6736(10)61029-X.

Roseff SD, 2009, Sickle Cell Disease: A Review. Immunohematol J Blood Group Serol Educ, 25(2):67-74.

GBD, 2015, Mortality and Causes of Death Collaborators 2016, Global, Regional, and National Life Expectancy, All-cause Mortality, and Cause-specific Mortality for 249 Causes of Death, 1980-2015: A Systematic Analysis for the Global Burden of Disease Study 2015. Lancet, 388(10053):1459-544. DOI 10.1016/S0140-6736(16)31012-1.

National Heart, Lung and Blood Institute, 2016, How is Sickle Cell Disease Treated? Amended; 2017. Available from: https://www.nhlbi.nih.gov/health-topics/sickle-celldisease. [Last accessed on 2019 May 24].

Hagedorn TJ, Grosse IR, Krishnamurty S, 2015, A Concept Ideation Framework for Medical Device Design. J Biomed Inform, 55:218-30. DOI 10.1016/j.jbi.2015.04.010.

Arntzen-Bechina A, Leguy C, 2007, A Model of Knowledge Sharing in Biomedical Engineering: Challenges and Requirements. J Bus Chem, 4(1):27-43.

Wong KK, Tu JY, Sun Z, et al., 2013, Methods in Research and Development of Biomedical Devices. Singapore: World Scientific Publishing Co.

Bradbury TJ, Gaylo CM, Fairweather JA, et al., 2004, System and Method for Rapidly Customizing Design, Manufacture and/or Selection of Biomedical Devices. U.S. Patent Number 6772026.

Chu C, Graf G, Rosen DW, 2008, Design for Additive Manufacturing of Cellular Structures. Comput Aided Des Appl, 5(5):686-96.

Bourell DL, Beaman JB, Leu MC, et al., 2009, A Brief History of Additive Manufacturing and the 2009 Roadmap for Additive Manufacturing: Looking Back and Looking Ahead. In: Proceedings of the US-Turkey Workshop on Rapid Technologies, pp. 24-25.

Gibson I, Rosen D, Stucker B, 2015, Additive Manufacturing Technologies: 3D Printing, Rapid Prototyping, and Direct Digital Manufacturing. New York: Springer. DOI 10.1007/978-1-4939-2113-3.

Rosen DW, 2007, Computer-Aided Design for Additive Manufacturing of Cellular Structures. Comput Aided Des Appl, 4(5):585-94.

Wong KV, Hernandez A, 2012, A Review of Additive Manufacturing. ISRN Mech Eng, 2012:1-10.

Noorani RI, 2006, Rapid Prototyping: Principles and Applications. Los Angeles: John Wiley and Sons.

Ponche R, Hascoet JY, Kerbrat O, et al., 2012, A New Global Approach to Design for Additive Manufacturing. Virtual Phys Prototyp, 7(2):93-105.

Mcdonald JC, Duffy DC, Anderson JR, et al., 2000, Fabrication of Microfluidic Systems in Poly (Dimethylsiloxane). Electrophoresis, 21(1):27-40. DOI 10.1002/(sici)1522-2683 (20000101)21:1<27:aid-elps27>3.0.co;2-c.

Kaihara S, Borenstein J, Koka R, et al., 2000, Silicon Micromachining to Tissue Engineer Branched Vascular Channels for Liver Fabrication. Tissue Eng, 6:105-17. DOI 10.1089/107632700320739.

Shin M, Matsuda K, Ishii O, et al., 2004, Endothelialized Networks with a Vascular Geometry in Microfabricated Poly(Dimethyl Siloxane). Biomed Microdevices, 6:269-78. DOI 10.1023/b: bmmd.0000048559.29932.27.

Ozbolat V, Dey M, Ayan B, et al., 2018, 3D Printing of PDMS

Improves Its Mechanical and Cell Adhesion Properties. ACS Biomater Sci Eng, 4(2):682-93. DOI 10.1021/ acsbiomaterials.7b00646.

Lachaux J, Alcaine C, Gómez-Escoda B, et al., 2017, Thermoplastic Elastomer with Advanced Hydrophilization and Bonding Performances for Rapid (30 s) and Easy Molding of Microfluidic Devices. Lap Chip, 17:2581-94. DOI 10.1039/c7lc00488e.

Lu Z, Jiang X, Zuo X, et al., 2016, Improvement of Cytocompatibility of 3D-printing Resins for Endothelial Cell Adhesion. RSC Adv, 6(104):102381-8. DOI 10.1039/c6ra20700f.

Lenoir L, Segonds F, Bartolucci P, et al., 2019, A Methodology to Product a Complex Vascular Geometry Using Mainly Additive Manufacturing. Paris: CONFERE.

Peace D, 2017, Chapter 1: Neuro-ophthalmic anatomy. Fastest Otolaryngol Ophthalmol Insight Eng, 1: 1-8.

Vignon I, 2018, Carotid Angiography-MRI Model. Inria: Internal Report.

DOI: http://dx.doi.org/10.18063/ijb.v5i2.238


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