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Bioprinting with human stem cells-laden alginate-gelatin bioink and bioactive glass for tissue engineering

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Krishna C. R. Kolan, Julie A. Semon, Bradley Bromet, Delbert E. Day, Ming C. Leu


Three-dimensional (3D) bioprinting technologies have shown great potential in fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds with 12x12x1 mm3 in overall dimensions are physically characterized, and the glass dissolution from PCL/glass composite over a period of 28 days is studied. Alginate-gelatin composite is used as a bioink to suspend ASCs, and scaffolds are then bioprinted in different configurations: Bioink only, PCL+bioink, and PCL/glass+bioink, to investigate ASC viability. The results indicate that the solvent-based bioprinting process to fabricate 3D tissue models with bioactive glasses provides more than 80% cell viability immediately after printing and more than 60% viability after 7 days in normal culture conditions. The feasibility of modifying bioink with 13-93B3 glass for bioprinting is also investigated and the results are discussed.


Bioprinting; Alginate-Gelatin Hydrogel; Borate Bioactive Glass; Polymer/Bioactive Glass Composite; Human Adipose-Derived Stem Cells

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