Development and Characterisation of a Photocurable Alginate Bioink for 3D Bioprinting

Mishbak H. H, Glen Cooper, Paulo Jorge Da Silva Bartolo

Article ID: 189
Vol 5, Issue 2, 2019, Pages

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Alginate is a biocompatible material suitable for biomedical applications, which can be processed under mild conditions upon irradiation.  This paper investigates the preparation and the rheological behaviour of different pre-polymerised and polymerised alginate-methacrylate systems for 3D photopolymerisation bioprinting. The effect of the functionalization time on the mechanical, morphological, swelling and degradation characteristics of crosslinked alginate hydrogel is also discussed.  Alginate was chemically-modified with methacrylate groups and different reaction times considered. Photocurable alginate systems were prepared  by dissolving functionalized alginate with 0.5-1.5% photoinitiator solution and crosslinked by ultraviolet (UV) light (8 mW/cm2).


3D bioprinting, Alginate, Hydrogel, Photopolymerisation

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Augst, Alexander D., Hyun Joon Kong, and David J. Mooney. 2006. “Alginate Hydrogels as Biomaterials.” Macromolecular Bioscience 6 (8): 623–33.

Axpe, Eneko, and Michelle L Oyen. 2016. “Applications of Alginate-Based Bioinks in 3D Bioprinting.” Edited by Hermann Ehrlich. International Journal of Molecular Sciences 17 (12). MDPI: 1976.

Banerjee, Akhilesh, Manish Arha, Soumitra Choudhary, Randolph S Ashton, Surita R Bhatia, David V Schaffer, and Ravi S Kane. 2009. “The Influence of Hydrogel Modulus on the Proliferation and Differentiation of Encapsulated Neural Stem Cells.” Biomaterials 30 (27): 4695–99.

Bartolo, P J, and E Lenz. 2006. “Computer Simulation of Stereolithographic Curing Reactions: Phenomenological versus Mechanistic Approaches.” CIRP Annals 55 (1): 221–25.

Benton, Julie A, Cole A DeForest, Vani Vivekanandan, and Kristi S Anseth. 2009. “Photocrosslinking of Gelatin Macromers to Synthesize Porous Hydrogels That Promote Valvular Interstitial Cell Function.” Tissue Engineering Part A 15 (11). Mary Ann Liebert, Inc., publishers: 3221–30.

Bonino, Christopher a., Julia E. Samorezov, Oju Jeon, Eben Alsberg, and Saad a. Khan. 2011. “Real-Time in Situ Rheology of Alginate Hydrogel Photocrosslinking.” Soft Matter 7 (24): 11510.

Daemi, H., and M. Barikani. 2012. “Synthesis and Characterization of Calcium Alginate Nanoparticles, Sodium Homopolymannuronate Salt and Its Calcium Nanoparticles.” Scientia Iranica 19 (6). Elsevier B.V.: 2023–28.

Hermansson, Elin, Erich Schuster, Lars Lindgren, Annika Altskär, and Anna Ström. 2016. “Impact of Solvent Quality on the Network Strength and Structure of Alginate Gels.” Carbohydrate Polymers 144. Elsevier: 289–96.

Jeon, Oju, Kamal H. Bouhadir, Joseph M. Mansour, and Eben Alsberg. 2009. “Photocrosslinked Alginate Hydrogels with Tunable Biodegradation Rates and Mechanical Properties.” Biomaterials 30 (14). Elsevier Ltd: 2724–34.

Lam, Carlos, and Stephan A Jefferis. 2014. “Interpretation of Viscometer Test Results for Polymer Support Fluids.” In Tunneling and Underground Construction, 439–49.

Matias, Joao Manuel, Paulo Jorge Bartolo, and António Vilela Pontes. 2009. “Modeling and Simulation of Photofabrication Processes Using Unsaturated Polyester Resins.” Journal of Applied Polymer Science 114 (6). Wiley Online Library: 3673–85.

Moral, Çiğdem Kıvılcımdan, Özdemir Doğan, and Faika Dilek Sanin. 2013. “Comparison of Chemical Fractionation Method and 1H-NMR Spectroscopy in Measuring the Monomer Block Distribution of Algal Alginates.” Journal of Polymer Engineering 33 (3). De Gruyter: 239–46.

Müller, Michael, Ece Öztürk, Øystein Arlov, Paul Gatenholm, and Marcy Zenobi-Wong. 2017. “Alginate Sulfate--Nanocellulose Bioinks for Cartilage Bioprinting Applications.” Annals of Biomedical Engineering 45 (1): 210–23.

Nicodemus, Garret D, and Stephanie J Bryant. 2008. “Cell Encapsulation in Biodegradable Hydrogels for Tissue Engineering Applications.” Tissue Engineering Part B: Reviews 14 (2). Mary Ann Liebert, Inc., publishers: 149–65.

Ouwerx, C, N Velings, M.M Mestdagh, and M.A.V Axelos. 1998. “Physico-Chemical Properties and Rheology of Alginate Gel Beads Formed with Various Divalent Cations.” Polymer Gels and Networks 6 (5): 393–408.

Pawar, Amol A, Gabriel Saada, Ido Cooperstein, Liraz Larush, Joshua A Jackman, Seyed R Tabaei, Nam-Joon Cho, and Shlomo Magdassi. 2016. “High-Performance 3D Printing of Hydrogels by Water-Dispersible Photoinitiator Nanoparticles.” Science Advances 2 (4). American Association for the Advancement of Science: e1501381–e1501381.

Pawar, Siddhesh N, and Kevin J Edgar. 2012. “Alginate Derivatization: A Review of Chemistry, Properties and Applications.” Biomaterials 33 (11): 3279–3305.

Pereira, Rúben, Anabela Carvalho, Daniela C. Vaz, M.H. Gil, Ausenda Mendes, and Paulo Bártolo. 2013. “Development of Novel Alginate Based Hydrogel Films for Wound Healing Applications.” International Journal of Biological Macromolecules 52 (October 2015). Elsevier B.V.: 221–30.

Pereira, Rúben F., and Paulo J. Bártolo. 2015a. “3D Bioprinting of Photocrosslinkable Hydrogel Constructs.” Journal of Applied Polymer Science 132 (48): n/a-n/a.

Pereira, Rúben F, and Paulo J Bártolo. 2015b. “3D Photo-Fabrication for Tissue Engineering and Drug Delivery.” Engineering 1 (1): 90–112.

Pereira, Rúben F, Aureliana Sousa, Cristina C Barrias, Paulo J Bártolo, and Pedro L Granja. 2018. “A Single-Component Hydrogel Bioink for Bioprinting of Bioengineered 3D Constructs for Dermal Tissue Engineering.” Materials Horizons 5 (6). Royal Society of Chemistry: 1100–1111.

Pereira, Rúben, Ana Tojeira, Daniela C. Vaz, Ausenda Mendes, and Paulo Bártolo. 2011. “Preparation and Characterization of Films Based on Alginate and Aloe Vera.” International Journal of Polymer Analysis and Characterization 16 (7): 449–64.

Smeds, K, A Pfister-Serres, D Miki, K A Dastghieb, M Inoue, D L Hatchell, and M W Grinstaff. 2001. “Novel Photocrosslinkable Polysaccharides for In Situ Hydrogel Formation.” J. Biomed. Mat. Res. 54 (July): 115–21.

Smeriglio, Piera, Janice H Lai, Fan Yang, and Nidhi Bhutani. 2015. “3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation.” Journal of Visualized Experiments : JoVE, no. 104 (October). MyJove Corporation: 53085.

Spadari, Cristina de Castro, Luciana B Lopes, and Kelly Ishida. 2017. “Potential Use of Alginate-Based Carriers as Antifungal Delivery System.” Frontiers in Microbiology 8. Frontiers: 97.

Stagnaro, P, I Schizzi, R Utzeri, E Marsano, and M Castellano. 2018. “Alginate-Polymethacrylate Hybrid Hydrogels for Potential Osteochondral Tissue Regeneration.” Carbohydrate Polymers 185: 56–62.

Sudhakar, C K, Nitish Upadhyay, Ankush Jain, Arpit Verma, R Narayana Charyulu, and Sanjay Jain. 2015. “Chapter 5 - Hydrogels—Promising Candidates for Tissue Engineering.” In , edited by Sabu Thomas, Yves Grohens, and Neethu B T - Nanotechnology Applications for Tissue Engineering Ninan, 77–94. Oxford: William Andrew Publishing.

Szekalska, Marta, Agata Puciłowska, Emilia Szymańska, Patrycja Ciosek, and Katarzyna Winnicka. 2016. “Alginate: Current Use and Future Perspectives in Pharmaceutical and Biomedical Applications.” International Journal of Polymer Science 2016. Hindawi.

Vyas, Cian, Rúben Pereira, Boyang Huang, Fengyuan Liu, Weiguang Wang, and Paulo Bartolo. 2017. “Engineering the Vasculature with Additive Manufacturing.” Current Opinion in Biomedical Engineering 2. Elsevier: 1–13.

Wang, Weiguang, Guilherme Caetano, William Stephen Ambler, Jonny James Blaker, Marco Andrey Frade, Parthasarathi Mandal, Carl Diver, and Paulo Bártolo. 2016. “Enhancing the Hydrophilicity and Cell Attachment of 3D Printed PCL/Graphene Scaffolds for Bone Tissue Engineering.” Materials 9 (12). Multidisciplinary Digital Publishing Institute: 992.

Yang, Xingchen, Zhenhui Lu, Huayu Wu, Wei Li, Li Zheng, and Jinmin Zhao. 2018. “Collagen-Alginate as Bioink for Three-Dimensional (3D) Cell Printing Based Cartilage Tissue Engineering.” Materials Science and Engineering: C 83: 195–201.

You, Fu, B Frank Eames, and Xiongbiao Chen. 2017. “Application of Extrusion-Based Hydrogel Bioprinting for Cartilage Tissue Engineering.” International Journal of Molecular Sciences .



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