Open Journal Systems





Near-field electrospinning of a polymer/bioactive glass composite to fabricate 3D biomimetic structures

VIEWS - 400 (Abstract) 114 (PDF)
Krishna Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, Delbert E. Day, Ming C. Leu

Abstract


Bioactive glasses have recently gained attention in tissue engineering and three-dimensional (3D) bioprinting because of their ability to enhance angiogenesis. Some challenges for developing biological tissues with bioactive glasses include incorporation of glass particles and achieving a 3D architecture mimicking natural tissues. In this study, we investigate the fabrication of scaffolds with a polymer/bioactive glass composite using near-field electrospinning (NFES). An overall controlled 3D scaffold with pores, containing random fibers, is created and aimed to provide superior cell proliferation. Highly angiogenic borate bioactive glass (13-93B3) in 20 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using the NFES technique. Scaffolds measuring 5 mm × 5 mm × 0.2 mm3 in overall dimensions were seeded with human adipose-derived mesenchymal stem cells to investigate the cell viability. The cell viability on PCL and PCL+glass scaffolds fabricated using NFES technique and 3D printing is compared and discussed. The results indicated higher cell proliferation on 3D biomimetic scaffolds fabricated by NFES technique


Keywords


electrospinning; polymer borate glass composite; 3D scaffold;

Full Text:

PDF

References


Bružauskaitė I, Bironaitė D, Bagdonas E, et al., 2016, Scaffolds and cells for tissue regeneration: different scaffold pore sizes-different cell effects. Cytotechnology vol.68(3):355–69. http://dx.doi.org/10.1007/s10616-015-9895-4

Kolan K C R, Thomas A, Leu M C, et al., 2015, In vitro assessment of laser sintered bioactive glass scaffolds with different pore geometries. Rapid Prototyp. J. vol.21(2):152–158. http://dx.doi.org/10.1108/RPJ-12-2014-0175

Kolan K C R, Leu M C, Hilmas G E, et al., 2013, Effect of architecture and porosity on mechanical properties of borate glass scaffolds made by selective laser sintering. 24th Int. SFF Symp. - An Addit. Manuf. Conf. SFF 2013 :816–826.

Melchels F P W, Barradas A M C, Van Blitterswijk C a., et al., 2010, Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing. Acta Biomater. vol.6(11):4208–4217. http://dx.doi.org/10.1016/j.actbio.2010.06.012

Fu Q, Saiz E, Tomsia A P, 2011, Direct ink writing of highly porous and strong glass scaffolds for load-bearing bone defects repair and regeneration. Acta Biomater. vol.7(10):3547–3554. http://dx.doi.org/10.1016/j.actbio.2011.06.030

Ozbolat I T, Hospodiuk M, 2016, Current advances and future perspectives in extrusion-based bioprinting. Biomaterials vol.76:321–343. http://dx.doi.org/10.1016/j.biomaterials.2015.10.076

Li Y, Bou-Akl T, 2016, Electrospinning in Tissue Engineering. Electrospinning - Mater. Tech. Biomed. Appl. . http://dx.doi.org/10.5772/65836

Giannitelli S M, Mozetic P, Trombetta M, et al., 2015, Combined additive manufacturing approaches in tissue engineering. Acta Biomater. vol.24:1–11. http://dx.doi.org/10.1016/j.actbio.2015.06.032

Daoheng S, Chieh C, Sha, et al., 2006, Near-Field Electrospinning. Nano Lett., vol.6(4):839-842. http://dx.doi.org/10.1021/NL0602701

He X-X, Zheng J, Yu G-F, et al., 2017, Near-Field Electrospinning: Progress and Applications. J. Phys. Chem. C vol.121(16):8663–8678. http://dx.doi.org/10.1021/acs.jpcc.6b12783

Moura D, Souza M T, Liverani L, et al., 2017, Development of a bioactive glass-polymer composite for wound healing applications. Mater. Sci. Eng. C vol.76:224–232. http://dx.doi.org/10.1016/j.msec.2017.03.037

Liverani L, Lacina J, Roether J A, et al., 2018, Incorporation of bioactive glass nanoparticles in electrospun PCL/chitosan fibers by using benign solvents. Bioact. Mater. vol.3(1):55–63. http://dx.doi.org/10.1016/J.BIOACTMAT.2017.05.003

Rahaman M N, Day D E, Sonny Bal B, et al., 2011, Bioactive glass in tissue engineering. Acta Biomater. vol.7(6):2355–2373. http://dx.doi.org/10.1016/j.actbio.2011.03.016

Murphy C, Kolan K, Li W, et al., 2017, 3D bioprinting of stem cells and polymer/bioactive glass composite scaffolds for tissue engineering. Int. J. Bioprinting vol.3(1):54–64. http://dx.doi.org/10.18063/IJB.2017.01.005

Kolan K, Liu Y, Baldridge J, et al., 2017, Solvent Based 3D Printing of Biopolymer/Bioactive Glass Composite and Hydrogel for Tissue Engineering Applications. Procedia CIRP vol.65:38–43. http://dx.doi.org/10.1016/J.PROCIR.2017.04.022

Boyde A, 2003, Improved digital SEM of cancellous bone: scanning direction of detection, through focus for in-focus and sample orientation. J. Anat. vol.202(2):183–94. http://dx.doi.org/10.1046/J.1469-7580.2003.00146.X

Jung S, 2010, Borate based bioactive glass scaffolds for hard and soft tissue engineering. Dr. Diss.




DOI: http://dx.doi.org/10.18063/ijb.v5i1.163

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Krishna Kolan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.