Near-field electrospinning of a polymer/bioactive glass composite to fabricate 3D biomimetic structures

Krishna Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, Delbert E. Day, Ming C. Leu

Article ID: 163
Vol 5, Issue 1, 2019, Article identifier:163

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Abstract


Bioactive glasses have recently gained attention in tissue engineering and three-dimensional (3D) bioprinting because of their ability to enhance angiogenesis. Some challenges for developing biological tissues with bioactive glasses include incorporation of glass particles and achieving a 3D architecture mimicking natural tissues. In this study, we investigate the fabrication of scaffolds with a polymer/bioactive glass composite using near-field electrospinning (NFES). An overall controlled 3D scaffold with pores, containing random fibers, is created and aimed to provide superior cell proliferation. Highly angiogenic borate bioactive glass (13-93B3) in 20 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using the NFES technique. Scaffolds measuring 5 mm × 5 mm × 0.2 mm3 in overall dimensions were seeded with human adipose-derived mesenchymal stem cells to investigate the cell viability. The cell viability on PCL and PCL+glass scaffolds fabricated using NFES technique and 3D printing is compared and discussed. The results indicated higher cell proliferation on 3D biomimetic scaffolds fabricated by NFES technique.


Keywords


Near-field electrospinning; three-dimensional biomimetic scaffold; polycaprolactone; polymer/bioactive glass composite; borate bioactive glass; human adipose-derived stem cells

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References


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DOI: http://dx.doi.org/10.18063/ijb.v5i1.163

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