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Near-field electrospinning of a polymer/bioactive glass composite to fabricate 3D biomimetic structures

VIEWS - 61 (Abstract) 15 (PDF)
Krishna Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, Delbert E. Day, Ming C. Leu

Abstract


Bioactive glasses have recently gained attention in tissue engineering and three-dimensional (3D) bioprinting because of their ability to enhance angiogenesis. Some challenges for developing biological tissues with bioactive glasses include incorporation of glass particles and achieving a 3D architecture mimicking natural tissues. In this study, we investigate the fabrication of scaffolds with a polymer/bioactive glass composite using near-field electrospinning (NFES). An overall controlled 3D scaffold with pores, containing random fibers, is created and aimed to provide superior cell proliferation. Highly angiogenic borate bioactive glass (13-93B3) in 20 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using the NFES technique. Scaffolds measuring 5 mm × 5 mm × 0.2 mm3 in overall dimensions were seeded with human adipose-derived mesenchymal stem cells to investigate the cell viability. The cell viability on PCL and PCL+glass scaffolds fabricated using NFES technique and 3D printing is compared and discussed. The results indicated higher cell proliferation on 3D biomimetic scaffolds fabricated by NFES technique


Keywords


electrospinning; polymer borate glass composite; 3D scaffold;

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References


Bružauskaitė I, Bironaitė D, Bagdonas E, et al., 2016, Scaffolds and cells for tissue regeneration: different scaffold pore sizes-different cell effects. Cytotechnology vol.68(3):355–69. http://dx.doi.org/10.1007/s10616-015-9895-4

Kolan K C R, Thomas A, Leu M C, et al., 2015, In vitro assessment of laser sintered bioactive glass scaffolds with different pore geometries. Rapid Prototyp. J. vol.21(2):152–158. http://dx.doi.org/10.1108/RPJ-12-2014-0175

Kolan K C R, Leu M C, Hilmas G E, et al., 2013, Effect of architecture and porosity on mechanical properties of borate glass scaffolds made by selective laser sintering. 24th Int. SFF Symp. - An Addit. Manuf. Conf. SFF 2013 :816–826.

Melchels F P W, Barradas A M C, Van Blitterswijk C a., et al., 2010, Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing. Acta Biomater. vol.6(11):4208–4217. http://dx.doi.org/10.1016/j.actbio.2010.06.012

Fu Q, Saiz E, Tomsia A P, 2011, Direct ink writing of highly porous and strong glass scaffolds for load-bearing bone defects repair and regeneration. Acta Biomater. vol.7(10):3547–3554. http://dx.doi.org/10.1016/j.actbio.2011.06.030

Ozbolat I T, Hospodiuk M, 2016, Current advances and future perspectives in extrusion-based bioprinting. Biomaterials vol.76:321–343. http://dx.doi.org/10.1016/j.biomaterials.2015.10.076

Li Y, Bou-Akl T, 2016, Electrospinning in Tissue Engineering. Electrospinning - Mater. Tech. Biomed. Appl. . http://dx.doi.org/10.5772/65836

Giannitelli S M, Mozetic P, Trombetta M, et al., 2015, Combined additive manufacturing approaches in tissue engineering. Acta Biomater. vol.24:1–11. http://dx.doi.org/10.1016/j.actbio.2015.06.032

Daoheng S, Chieh C, Sha, et al., 2006, Near-Field Electrospinning. Nano Lett., vol.6(4):839-842. http://dx.doi.org/10.1021/NL0602701

He X-X, Zheng J, Yu G-F, et al., 2017, Near-Field Electrospinning: Progress and Applications. J. Phys. Chem. C vol.121(16):8663–8678. http://dx.doi.org/10.1021/acs.jpcc.6b12783

Moura D, Souza M T, Liverani L, et al., 2017, Development of a bioactive glass-polymer composite for wound healing applications. Mater. Sci. Eng. C vol.76:224–232. http://dx.doi.org/10.1016/j.msec.2017.03.037

Liverani L, Lacina J, Roether J A, et al., 2018, Incorporation of bioactive glass nanoparticles in electrospun PCL/chitosan fibers by using benign solvents. Bioact. Mater. vol.3(1):55–63. http://dx.doi.org/10.1016/J.BIOACTMAT.2017.05.003

Rahaman M N, Day D E, Sonny Bal B, et al., 2011, Bioactive glass in tissue engineering. Acta Biomater. vol.7(6):2355–2373. http://dx.doi.org/10.1016/j.actbio.2011.03.016

Murphy C, Kolan K, Li W, et al., 2017, 3D bioprinting of stem cells and polymer/bioactive glass composite scaffolds for tissue engineering. Int. J. Bioprinting vol.3(1):54–64. http://dx.doi.org/10.18063/IJB.2017.01.005

Kolan K, Liu Y, Baldridge J, et al., 2017, Solvent Based 3D Printing of Biopolymer/Bioactive Glass Composite and Hydrogel for Tissue Engineering Applications. Procedia CIRP vol.65:38–43. http://dx.doi.org/10.1016/J.PROCIR.2017.04.022

Boyde A, 2003, Improved digital SEM of cancellous bone: scanning direction of detection, through focus for in-focus and sample orientation. J. Anat. vol.202(2):183–94. http://dx.doi.org/10.1046/J.1469-7580.2003.00146.X

Jung S, 2010, Borate based bioactive glass scaffolds for hard and soft tissue engineering. Dr. Diss.




DOI: http://dx.doi.org/10.18063/ijb.v5i1.163

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