Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs

VIEWS - 164 (Abstract) 34 (PDF)
Hongtao Liang, Jiankang He, Jinke Chang, Bing Zhang, Dichen Li

Abstract


Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements. However, it is still challenging to print cell-laden 3D structures simultaneously with high resolution and high cell viability. Here a coaxial nozzle-assisted electrohydrodynamic cell printing strategy was developed to fabricate living 3D cell-laden constructs. Critical process parameters such as feeding rate and stage moving speed were evaluated to achieve smaller hydrogel filaments. The effect of CaCl2 feeding rate on the printing of 3D alginate hydrogel constructs was also investigated. The results indicated that the presented strategy can print 3D hydrogel structures with relatively uniform filament dimension (about 80 μm) and cell distribution. The viability of the encapsulated cells was over 90%. We envision that the coaxial nozzle-assisted electrohydrodynamic printing will become a promising cell printing strategy to advance biomedical innovations.


Keywords


Electrohydrodynamic printing; cell printing; bioprinting; biofabrication; tissue engineering

Full Text:

PDF

References


Kang H-W, Lee S J, Ko I K, et al., 2016, A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechno, 34(3): 313–319. http://dx.doi.org/10.1038/nbt.3413

Jordan S M, Kelly R S, Michael T Y, et al., 2012, Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nat Mater, 11(9): 768–774. http://dx.doi.org/10.1038/nmat335733

Falguni P, Jinah J, Dong-Heon H, et al., 2014, Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun, 5: 3935. http://dx.doi.org/10.1038/ncomms4935

Sean V M, Anthony A, 2014, 3D bioprinting of tissues and organs. Nat Biotechnol, 32(8): 773–785. http://dx.doi.org/10.1038/nbt.2958

Malda J, Jetze V, Ferry P M, et al., 2013, 25th Anniversary article: Engineering hydrogels for biofabrication. Adv Mater, 25(36): 5011–5028. http://dx.doi.org/10.1002/adma.201302042

Thomas B, Mieke V, Jorg S, et al., 2012, A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering. Biomaterials, 33(26): 6020–6041. http://dx.doi.org/10.1016/j.biomaterials.2012.04.050

Xu T, Zhao W Z, Zhu J M, et al., 2013, Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology. Biomaterials, 34(1): 130–139. http://dx.doi.org/10.1016/j.biomaterials.2012.09.035

Lothar K, Andrea D, Sabrina S, et al., 2012, Skin tissue generation by laser bioprinting. Biotechnology, 109(7): 1855–1863. http://dx.doi.org/10.1002/bit.24455

Zhu W, Ma X Y, Gou M L, et al., 2016, 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol, 40: 103–112. http://dx.doi.org/10.1016/j.copbio.2016.03.014

Ning LQ, Chen X B, 2017, A brief review of extrusion-based tissue scaffold bio-printing. Biotechnol J, 12(8): 1600671. http://dx.doi.org/10.1002/biot.201600671

Koo Y, Kim G, 2016, New strategy for enhancing in situ cell viability of cell-printing process via piezoelectric transducer-assisted three-dimensional printing. Biofabrication, 8(2): 025010. http://dx.doi.org/10.1088/1758-5090/8/2/025010

Zhang B, He J K, Li X, et al., 2016, Micro/nanoscale electrohydrodynamic printing: From 2D to 3D. Nanoscale, 8(34): 15376–15388. http://dx.doi.org/10.1039/c6nr04106j

He J K, Xu FY, Cao Y, et al., 2015, Towards microscale electrohydrodynamic three-dimensional printing. J Phys D Appl Phys, 49(5): 055504. http://dx.doi.org/10.1088/0022-3727/49/5/055504

He J K, Xu F Y, Dong R N, et al., 2016, Electrohydrodynamic 3D printing of microscale poly (ε-caprolactone) scaffolds with multi-walled carbon nanotubes. Biofabrication, 9(1): 015007. http://dx.doi.org/10.1088/1758-5090/aa53bc

Mao M, He J K, Li X, et al., 2017, The emerging frontiers and applications of high-resolution 3D printing. Micromachines, 8(4): 113. http://dx.doi.org/10.3390/mi8040113

Onses M S, Sutanto E, Ferreira P M, et al., 2015, Mechanisms, capabilities, and applications of high-resolution electrohydrodynamic jet printing. Small, 11(34): 4267–4266. http://dx.doi.org/10.1002/smll.201500593

Lee H, Seong B, Jang Y, et al., 2014, Direct alignment and patterning of silver nanowires by electrohydrodynamic jet printing. Small, 10(19): 3918–3922. http://dx.doi.org/10.1002/smll.201400936

Ahmad Z, Rasekh M, Edirisinghe M, 2010, Electrohydrodynamic direct writing of biomedical polymers and composites. Macromol Mater Eng, 295(4): 315–319. http://dx.doi.org/10.1002/mame.200900396

Parajuli D, Koomsap P, Parkhi A A, et al., 2016, Experimental investigation on process parameters of near-field deposition of electrispinning-based rapid prototyping. Virtual Phys Prototyp, 11(3):193–207. http://dx.doi.org/10.1080/17452759.2016.1210314

Wei C, Dong J, 2013, Direct fabrication of high-resolution three-dimensional polymeric scaffolds using electrohydrodynamic hot jet plotting. J Micromech Microeng, 23(2): 025017. http://dx.doi.org/10.1088/0960-1317/23/2/025017.

Zheng G, Sun L L, Wang X, et al., 2016, Electrohydrodynamic direct-writing microfiber patterns under stretching. Appl Phys A Mater Sci Process, 122(2): 1–9. http://dx.doi.org/10.1007/s00339-015-9584-3

Chanthakulchan A, Koomsap P, Parkhi, et al., 2015, Environmental effects in fiber fabrication using electrispinning-based rapid prototyping. Virtual Phys Prototyp, 10(4): 227–237. http://dx.doi.org/10.1080/17452759.2015.1112411

Chanthakulchan A, Koomsap P, Auyson K, et al., 2015, Development of an electrospinning-based rapid prototyping for scaffold fabrication, Rapid Prototyp J, 21(3): 329–339. http://dx.doi.org/10.1108/RPJ-11-2013-0119

Bisht GS, Ciulin C, Alireza M, et al., 2011, Controlled continuous patterning of polymeric nanofibers on three-dimensional substrates using low voltage near-field electrospinning. Nano Lett, 11(4): 1831–1837. http://dx.doi.org/10.1021/nl2006164

Li J L, Cai YL, Guo Y L, et al., 2014, Fabrication of three-dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E-jetting technique. J Biomed Mater Res B Appl Biomater, 102B(4): 651–658. http://dx.doi.org/10.1002/jbm.b.33043

Bu N, Huang Y G, Wang X M, et al., 2012, Continuous tunable and oriented nanofiber direct-written by mechano-electrospinning. Mater Manuf Process, 27(12): 1318–132. http://dx.doi.org/10.1080/10426914.2012.700145

Jayasinghe S N, 2013, Cell electrospinning: A novel tool for functionalizing fibres, scaffolds and membranes with living cells and other advanced materials for regenerative biology and medicine. Analyst, 138(8): 2215–2223. http://dx.doi.org/10.1039/c3an36599a

Zhao X, He J K, Xu F Y, et al., 2016, Electrohydrodynamic printing: A potential tool for high-resolution hydrogel/cell patterning. Virtual Phys Prototyp, 11 (1): 57–63. http://dx.doi.org/10.1080/17452759.2016.1139378

Ehler E, Jayasinghe S N, 2014, Cell electrospinning cardiac patches for tissue engineering the heart. Analyst, 139(18): 4449–4452. http://dx.doi.org/10.1039/c4an00766b

Jayasinghe S N, Qureshi, A N, Eagles, P A, et al., 2006, Electrohydrodynamic jet processing: An advanced electric-field-driven jetting phenomenon for processing living cells. Small, 2(2): 216–219. http://dx.doi.org/10.1002/smll.200500291

Gasperini L, Manigiglio D, Motta A, et al., 2015, An electrohydrodynamic bioprinter for alginate hydrogels containing living cells. Tissue Eng Part C Methods, 21(2): 123–132. http://dx.doi.org/10.1089./ten.tec.2014.0149

Yeo M, Ha J H, Lee H, et al., 2016, Fabrication of hASCs-laden structures using extrusion-based bioprinting supplemented with an electric field. Acta Biomater, 38: 33–43. http://dx.doi.org/10.1016/j.actbio.2016.04.017

He J K, Zhao X, Chang J K, et al., 2017, Microscale electrohydrodynamic bioprinting with high viability. Small: 1702626. http://dx.doi.org/10.1002/smll.201702626




DOI: http://dx.doi.org/10.18063/ijb.v4i1.127

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Hongtao Liang, Jiankang He, Jinke Chang, Bing Zhang, Dichen Li

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Recent Articles | About Journal | For Author | Fees | About Whioce

Copyright © Whioce Publishing Pte Ltd. All Rights Reserved.