Directed self-assembly software for single cell deposition

Samuel C. Sklare, Winona L. Richey, Benjamin T. Vinson, Douglas B. Chrisey

Article ID: 102
Vol 3, Issue 2, 2017, Article identifier:100-108

VIEWS - 1417 (Abstract) 216 (PDF)


Laser direct-write (LDW) bioprinting methods offer a diverse set of tools to design experiments, fabricate tissue constructs and to cellular microenvironments all in a CAD/CAM manner. To date, we have just scratched the surface of the system’s potential and for LDW to be utilized to its fullest, there are many distinct hardware and software components that must be integrated and communicate seamlessly. In this perspective article, we detail the development of novel graphical user interface (GUI) software to improve LDW capability and functionality. The main modules in the control software correspond to cell transfer, microbead fabrication, and micromachining. The modules make the control of each of these features, and the management of printing programs that utilize one or more features, to be facile. The software also addresses problems related to construct scale-up, print speed, experimental conditions, and management of sensor data. The control software and possibilities for integrated sensor data are presented.


CAD/CAM Bioprinting; Laser Direct-Write; Single-cell Printing; GUI software, machine learning

Full Text:



Curley J L, Sklare S C, Bowser D A, et al., 2016, Isolated node engineering of neuronal systems using laser direct write. Biofabrication vol.8(1): 15013.

Phamduy T B, Raof N A, Schiele N R, et al., 2012, Laser direct-write of single microbeads into spatially-ordered patterns. Biofabrication vol.4(2): 25006.

Phamduy T B, Sweat R S, Azimi M S, et al., 2015, Printing cancer cells into intact microvascular networks: A model for investigating cancer cell dynamics during angiogenesis. Integr Biol (Camb) vol.7(9): 1068–1078.

Sklare S C, Phamduy T B, Curly J L, et al., 2015, The power of CAD/CAM laser bioprinting at the single-cell level: Evolution of printing. In: Zhang L G, Fisher J P, Leong K W (eds). 3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine. London, UK: Elsevier. 79–103.

Schiele N R, Corr D T, Huang Y, et al., 2010, Laser-based direct-write techniques for cell printing. Biofabrication vol.2(3): 32001.

Doraiswamy A, Patz T, Narayan R J, et al., 2006, Two-dimensional differential adherence of neuroblasts in laser micromachined CAD/CAM agarose channels. Appl Surf Sci vol.252(13): 4748–4753.

Kingsley D M, Dias A D, Chrisey D B, et al., 2013, Single-step laser-based fabrication and patterning of cell-encapsulated alginate microbeads. Biofabrication vol.5(4): 45006.

Piqué A, Chrisey D B, Auyeung R C Y, et al., 1999, A novel laser transfer process for direct writing of electronic and sensor materials. Appl Phys A vol.69(Supp 1): S279–S284.

Smausz T, Hopp B, Kecskeméti G, et al., 2006, Study on metal microparticle content of the material transferred with Absorbing Film Assisted Laser Induced Forward Transfer when using silver absorbing layer. Appl Surf Sci vol.252(13): 4738–4742.

Ringeisen B R, Othon C M, Wu X, et al., 2010, Biological laser printing (BioLP) for high resolution cell deposition. In: Ringeisen BR, Spargo BJ, Wu PK (eds). Cell and Organ Printing. Dordrecht, Netherlands: Springer. 81–93.

Doraiswamy A, Narayan R J, Lippert T, et al., 2006, Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer. Appl Surf Sci vol.252(13): 4743–4747.

Guillemot F, Souquet A, Catros S, et al., 2010, Laser-assisted cell printing: principle, physical parameters versus cell fate and perspectives in tissue engineering. Nanomedicine (Lond) vol.5(3): 507–515.

Liberski A R, Delaney J T, and Schubert U S, 2011, “One cell–one well”: A new approach to inkjet printing single cell microarrays. ACS Comb Sci vol.13(2): 190–195.

Patz T M, Doraiswamy A, Narayan R J, et al., 2006, Three-dimensional direct writing of B35 neuronal cells. J Biomed Mater Res Part B–Applied Biomater vol.78B(1): 124–130.

Guillemot F, Souquet A, Catros S, et al., 2010, High-throughput laser printing of cells and biomaterials for tissue engineering. Acta Biomater vol.6(7): 2494–2500.

Saunders R and, Derby B, 2014, Inkjet printing biomaterials for tissue engineering: Bioprinting. Int Mater Rev vol.59(8):430–448.

Yan J, Huang Y, and Chrisey DB, 2013, Laser-assisted printing of alginate long tubes and annular constructs. Biofabrication vol.5(1): 15002.

Gronle M, Lyda W, Wilke M, et al. 2014, itom: An open source metrology, automation, and data evaluation software. Appl Opt vol.53(14): 2974–2982.

Binder J M, Stark A, Tomek N, et al. 2017, Qudi: A modular python suite for experiment control and data processing. SoftwareX vol.6: 85–90.



  • There are currently no refbacks.

Copyright (c) 2017 Samuel C Sklare, Winona Richey, Benjamin T Vinson, Doug B Chrisey

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.