Electrospun 3D multi-scale fibrous scaffold for enhanced human dermal fibroblast infiltration

Wen Shing Leong, Shu Cheng Wu, KeeWoei Ng, Lay Poh Tan

Article ID: 02002
Vol 2, Issue 1, 2016, Article identifier:81-92

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Abstract


Electrospun polymeric nanofibrous scaffold possesses significant potential in the field of tissue engineering due to its extracellular matrix mimicking topographical features that modulate a variety of key cellular activities. However, traditional two dimensional (2D) electrospun scaffolds are generally close-packed fiber mats which prohibit cell infiltration and proliferation. Consequently, the applications of electrospun scaffolds in regenerative medicine is limited. In this study, we detail the use of a needle collector to fabricate 3D electrospun poly-ε-caprolactone (PCL) scaffolds with multi-scale fiber dimensions. The resultant pore size is 4 times larger than conventional 2D electrospun scaffolds with interweaving micro (3.3 ± 0.6µm) and nano (240 ± 50 nm) fibers. The scaffold was surface modified by grafting with gelatin molecules. It was found that surface modification significantly improved Human Dermal Fibroblasts (HDFs) cell infiltration throughout the 3D multi-scale scaffold. Even after an extended culture period of up to 28 days, cell proliferation was well supported in the surface-modified 3D multi-scale scaffold as confirmed by Ki67 staining. Extracellular matrix proteins secreted by the HDFs was evident on the 3D multi-scale PCL scaffold showing promising potential to facilitate tissue regeneration, in particular dermal tissue engineering.


Keywords


tissue engineering; 3D electrospinning scaffold; Human Dermal Fibroblasts; three dimensional scaffold; cell infiltration

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DOI: http://dx.doi.org/10.18063/IJB.2016.01.002

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