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Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

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Makoto Nakamura, Tanveer Ahmad Mir, Kenichi Arai, Satoru Ito, Toshiko Yoshida, Shintaroh Iwanaga, Hiromi Kitano, Chizuka Obara, Toshio Nikaido

Abstract


The fabrication of physiologically active tissue constructs from various tissue elements are vital for establishing integrated bioprinting and transfer printing techniques as vital tools for biomedical research. Physiologically functional tissues are hierarchically constructed from a variety of tissue subunits with different feature sizes and topographies. For example, skeletal muscles are composed of many muscle bundles, muscle fibers, and muscle cells respectively. The fundamental constituents of all types of muscle tissues include various sized blood vessels, and vascular related cells. Nature has designed the direction of all the aforementioned components to have unidirectional alignment, so that muscle contractions can effectively generate the mechanical functions.

In this study, we demonstrate a promising approach to fabricating such hierarchical tissues by applying bioprinting and a transfer patterning technique. Linear-patterned smooth muscle cells were obtained by culturing on the surface patterned discs, before being transferred onto the Matrigel substrate. The fiber-like tissues structures were successfully formed on the substrate after a few days of culturing; these are partially aligned smooth muscle cells. Additionally, stacked structures were also successfully fabricated using laminating printing technique. Our results indicate that bioprinting and transfer printing strategy of pre-cultured aligned muscular fiber-like tissues is very promising method to assemble tissue elements for biofabrication of hierarchical tissues.


Keywords


transfer cell printing; pre-cultured cell printing; laminating printing; fiber-like tissues

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References


Wilson WC Jr, Boland T. 2003, Cell and organ printing 1: protein and cell printers. Anat Rec, 272A, 491-496.

Boland T, Mironov V, Gutowska A, et al., 2003, Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels. Anat Rec, 272A, 497-502.

Mironov V, Boland T, Trusk T, et al., 2003, Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol, 21(4) 157-61.

Calvert P, 2007, Printing cells, Science 318, 208-9.

Jakab K, Damon B, Neagu A, Kachurin A, Forgacs G, 2006, Three-dimensional tissue constructs built by bioprinting. Biorheology, 43, 509-13.

Tsang VL, Bhatia SN. 2004 Three-dimensional tissue fabrication. Adv Drug Deliv Rev. 56(11):1635-47.

Serra P, Duocastella M, Fernández-Pradas JM, Morenza JL, 2010 Laser-Induced Forward Transfer: A Laser-Based Technique for Biomolecules Printing, Cell and OrganPrinting, eds.Ringersen BR, Spargo BJ, Wu P, (Springer) pp53-80

Ringeisen BR, Othon CM, Wu Xingjia, et al., 2010 Biological Laser Printing (BioLP) for High Resolution Cell Deposition, Cell and OrganPrinting, eds.Ringersen BR, Spargo BJ, Wu P, (Springer) pp81-94

Guillemot F, Guillotin B, Catros S, et al.,, 2010 High-Throughput Biological Laser Printing: Droplet Ejection Mechanism, Cell and OrganPrinting, eds.Ringersen BR, Spargo BJ, Wu P, (Springer) pp95-114.

Carlos C. Chang, Eugene D. et al.,, 2011 Direct-write bioprinting three-dimensional biohybrid systems for future regenerative therapies. J Biomed Mater Res B Appl Biomater. 98(1):160-70.

Guillemot F, Mironov V, Nakamura M, 2010, Bioprinting is coming of age: report from the International Conference on Bioprinting and Biofabrication in Bordeaux (3B'09), Biofabrication 2, 010201-7.

Bajaj P, Schweller RM, Khademhosseini A, et al.,. 2014 3D biofabrication strategies for tissue engineering and regenerative medicine. Annu Rev Biomed Eng. 16:247-76..

Collins SF. 2014 Bioprinting is changing regenerative medicine forever. Stem Cells Dev.;23 Suppl 1:79-82.

Ozbolat IT, Yu Y. 2013 Bioprinting toward organ fabrication: challenges and future trends.IEEE Trans Biomed Eng. 60(3):691-9.

Kobayashi A, Miyake H, Hattori H, et al.,. 2007 In vitro formation of capillary networks using optical lithographic techniques, Biochem Biophys Res Commun, 358 692-7

Yoshida T1, Komaki M, Hattori H, et al.,. 2010 Therapeutic angiogenesis by implantation of a capillary structure constituted of human adipose tissue microvascular endothelial cells. Arterioscler Thromb Vasc Biol.;30(7):1300-6..

Kitano H, Tada S, Mori T, et al.,. 2005 Correlation between the Structure of Water in the Vicinity of Carboxybetaine Polymers and Their Blood-Compatibility, Langmuir, 21(25) 11932-40

Tada S, Inaba C, Mizukami K, et al.,. 2009 Anti-Biofouling Properties of Polymers with a Carboxybetaine Moiety, Macromol. Biosci, 9 63-70

Kitano H, Kondo T, Kamada T, et al.,. 2011 Anti-biofouling properties of an amphoteric polymer brush constructed ona glass substrate , Colloids Surf B Biointerfaces, 88(2011) 455-62

Kitano H, Suzuki H, Kondo T, et al., 2011 Image Printing on the Surface of Anti-Biofouling Zwitterionic Polymer Brushes by Ion Beam Irradiation, Macromol.Biosci, 11 557-64

Nakamura M, Kobayashi A, Takagi F, et al.,. 2005, Biocompatible Inkjet Printing Technique For Designed Seeding Of Individual Living Cells. Tissue Eng. 11, 1658-1666.

Nishiyama Y, Nakamura M, Henmi C, et al.,. 2009, Development of three-dimensional bio-printer: Construction of cell supporting structures using hydrogel and state-of-the-art inkjet technology. J Biomech Eng 131 (3), 035001-6.

Nakamura M 2010, Reconstruction of biological three-dimensional tissues: Bioprinting and biofabrication using inkjet technology, Cell and OrganPrinting, eds.Ringersen BR, Spargo BJ, Wu P, (Springer) pp23-34

Arai K, Iwanaga S, Toda H, et al.,, 2011, Three-dimensional inkjet biofabrication based on designed images. Biofabrication. 3(3):034113.

Matsuda T, Inoue K, Sugawara T. 1990, Development of micropatterning technology for cultured cells. ASAIO Trans. 36(3):M559-62.

Iwanaga S, Akiyama Y, Kikuchi A, et al.,. 2005 Fabrication of a cell array on ultrathin hydrophilic polymer gels utilising electron beam irradiation and UV excimer laser ablation. Biomaterials. Sep;26(26):5395-404.

Lauer L, Klein C, Offenhausser 2001, A. Spot compliant neuronal networks by structure optimized micro-contact printing. Biomaterials 22, 1925-1932.

Klebe RJ. 1988, Cytoscribing: a method for micropositioning cells and the construction of two- and three-dimensional synthetic tissues. Exp Cell Res. 179(2):362-73.

Campbell PG, Miller ED, Fisher GW, et al.,. 2005, Engineered spatial patterns of FGF-2 immobilized on fibrin direct cell organization. Biomaterials. 26(33):6762-70.

Yang S., Leong KF, Du Z., Chua CK, 2002, The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. Tissue Eng. 8, 1-11.

Landers, R., Hubner, H., Schmelzeisen, R., Mülhaupt, R. 2002, Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. Biomaterials, 23, 4437-4447.

Griffith,LG., Naughton,G. 2002, Tissue engineering-current challenges and expanding opportunities. Science, 295, 1009-1014.

Wu PK, Ringeisen BR 2010, Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP). Biofabrication. 2(1):014111.

Okano T, Satoh S, Oka T, Matsuda T.1997, Tissue engineering of skeletal muscle. Highly dense, highly oriented hybrid muscular tissues biomimicking native tissues. ASAIO J. 43(5):M749-53.

Kanda K, Matsuda T. 1994, Mechanical stress-induced orientation and ultrastructural change of smooth muscle cells cultured in three-dimensional collagen lattices. Cell Transplant. 3(6):481-92.

Zimmermann WH, Schneiderbanger K, Schubert P, et al.,. 2002, Tissue engineering of a differentiated cardiac muscle construct. Circ Res. 90(2):223-30.

Eschenhagen T, Didié M, Heubach J, et al.,. 2002,Cardiac tissue engineering. Transpl Immunol. 9(2-4):315-21.

Eschenhagen T, Zimmermann WH. 2005, Engineering myocardial tissue. Circ Res. 97(12):1220-31.




DOI: http://dx.doi.org/10.18063/IJB.2015.01.007

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Copyright (c) 2017 Makoto Nakamura, Tanveer Ahmad Mir, Kenichi Arai, Satoru Ito, Toshiko Yoshida, Shintaroh Iwanaga, Hiromi Kitano, Chizuka Obara, Toshio Nikaido

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